Journal: BioMed Research International

Article Title: B7-H4 Expression in Precancerous Lesions of the Uterine Cervix

doi: 10.1155/2021/5857092

Figure Lengend Snippet: Correlation between B7-H4 expression and the number of infiltrating T lymphocytes and cytokines in patients with cervical disease.

Article Snippet: After blocking with 5% goat serum, slides were incubated with the following primary antibodies overnight at 4°C: rabbit anti-human Foxp3 (dilution 1 : 300, NB100-39002SS, Novus Biologicals, Littleton, CO, USA), mouse anti-human CD4 (dilution 1 : 200, NBP2-27216, Novus Biologicals), mouse anti-human CD8 alpha (dilution 1 : 200, NBP2-32836, Novus Biologicals), and rabbit anti-human IFN- γ (dilution 1 : 100, 8455P, Cell Signaling Technology).

Techniques: Expressing

Journal: BioMed Research International

Article Title: B7-H4 Expression in Precancerous Lesions of the Uterine Cervix

doi: 10.1155/2021/5857092

Figure Lengend Snippet: Double immunofluorescence-based staining of (a) CD4 + Foxp3 + and (b) interferon (IFN)- γ + CD8 + T cells. Magnification: ×200.

Article Snippet: After blocking with 5% goat serum, slides were incubated with the following primary antibodies overnight at 4°C: rabbit anti-human Foxp3 (dilution 1 : 300, NB100-39002SS, Novus Biologicals, Littleton, CO, USA), mouse anti-human CD4 (dilution 1 : 200, NBP2-27216, Novus Biologicals), mouse anti-human CD8 alpha (dilution 1 : 200, NBP2-32836, Novus Biologicals), and rabbit anti-human IFN- γ (dilution 1 : 100, 8455P, Cell Signaling Technology).

Techniques: Double Immunofluorescence Staining

Journal: iScience

Article Title: TRIAC disrupts cerebral thyroid hormone action via negative feedback and heterogenous distribution among organs

doi: 10.1016/j.isci.2023.107135

Figure Lengend Snippet: TRIAC effects on the hypothalamus-pituitary-thyroid axis in hypothyroid mice (A–D) Serum levels of 3,3′,5,5′-tetraiodothyronine (T4), 3,3′,5-triiodothyronine (T3), TRIAC, and thyroid-stimulating hormone (TSH). (E) Gross appearance of thyroid glands. The scale on the ruler is 1 mm. (F) Thyroid gland size was measured as thyroid area on photograph. (G–J) Histological images of thyroid glands stained using hematoxylin and eosin. Vehicle, PTU, PTU + TRIAC, n = 6 each; PTU + LT3, n = 5. BLQ, below the limit of quantitation. Concentrations of PTU, LT3, and TRIAC used were 100 μg/mL, 0.1 μg/mL, and 0.1 μg/mL, respectively. Data are represented as means ± SEM. Statistical analyses were performed using Student’s t -test for vehicle vs. PTU and the p value is presented as †††p < 0.001. ANOVA followed by the Tukey-Kramer test was used for comparisons among PTU, PTU + LT3, and PTU + TRIAC experiments and the p value is presented as ∗∗p < 0.01, and ∗∗∗p < 0.001.

Article Snippet: We measured serum free T4 levels using an FT3, FT4, TSH AccuLite VAST CLIA kit (Monobind Inc., Lake Forest, CA) with Spark (Tecan, Zurich, Switzerland).

Techniques: Staining, Quantitation Assay

Journal: Journal of Veterinary Internal Medicine

Article Title: Research Communications of the 29th ECVIM‐CA Congress

doi: 10.1111/jvim.15658

Figure Lengend Snippet:

Article Snippet: An automated free T4 analogue (fT4a) immunoassay (Immulite 2000 Veterinary Free T4, Siemens) has recently gained popularity.

Techniques: Isolation, Activity Assay, Blocking Assay, Biomarker Discovery, Comparison, Imaging, Clinical Proteomics, Marker, Membrane, Control, In Vitro, Infection, Real-time Polymerase Chain Reaction, Liquid Chromatography, Mass Spectrometry, Expressing, Diagnostic Assay, Immunopeptidomics, Sequencing, Concentration Assay